Thermo Scientific Slide-A-Lyzer Dialysis Flasks facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in sample volumes up to 250mL.
Slide-A-Lyzer Dialysis Flasks can be used to process sample volumes up to 250mL in 8 to 24 hours and are available in four molecular-weight cutoff (MWCO) varieties: 2K, 3.5K, 10K and 20K. The flask-style dialysis devices are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).
- Easy to use – Simply pipette or pour sample into flask and begin dialysis
- Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 250mL sample in 8 hours to overnight
- High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
- Multiple molecular-weight cutoffs – select the membrane MWCO that best suits your sample’s molecular weight
- Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color
Dialysis Membrane Specifications:
- Composition: Regenerated-cellulose
- Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
- Hydration Required Before Use: 30 seconds to 2 minutes
- Glycerol Content: Trace (10K MWCO content is 21%)
- Sulfur Content: 0.04 to 0.17%
- Heavy metals Content: Trace
Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.
The Slide-A-Lyzer Dialysis Flasks are designed to maximize ease of use for processing sample volumes of 150 to 250mL (230mL maximum for the 3.5K-membrane flasks). The flasks eliminate the risk of losing valuable samples associated with handling slippery dialysis tubing, which are difficult to knot or clip shut. Sample addition and removal are easily accomplished by pipetting or directly pouring the sample through the wide-mouth opening at the top of the flask.
With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.
|Thermo Scientific Slide-A-Lyzer Dialysis Flasks make sample loading and recovery easy. Attach supplied float-ring and hydrate membrane for 2 minutes. Pour sample into device. Remove air and cap. Dialyze for 8 hours to overnight (replace buffer after 2 and 5 hours). Pour out sample to recover.
|Effective sample dialysis with Thermo Scientific Slide-A-Lyzer Dialysis Flasks. Rates of removal of 1M NaCl from 200mL samples in 2K, 3.5K,10K, and 20K MWCO dialysis flasks. For each dialysis experiment, 200mL samples containing 1M NaCl were dialyzed at room temperature. The dialysis buffer (4L) was changed at the indicated times (triangles). The rate of NaCl removal was determined by measuring the conductivity at the indicated times (triangles; also at 41 hours for the 2K condition). Greater than 95% of NaCl was removed in 8 to 18 hours (41 hours for the 2K condition). These dialysis efficiencies, rates and recoveries are similar to conventional tubing-based dialysis.
Review of Dialysis Methods
All Dialysis Devices
Pre-formulated buffers for protein methods