Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Pierce Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.
Highlights:
- Specific – highly specific anti-HA monoclonal antibody enables high-yield and high-purity immunoprecipitation (IP)
- Fast – optimized protocol is completed in approximately 1 hour
- Low nonspecific binding – stable, pre-blocked beads provide highly purified HA-tagged proteins
- Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)
Product Details:
Thermo Scientific Pierce Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine, pH 2.0, 50mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2mg/mL Pierce HA peptide can be used. The protocol has been optimized for each of these conditions. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.
| Characteristics of Thermo Scientific Pierce Anti-HA Magnetic Beads. |
| Composition: |
High-affinity mouse IgG1 monoclonal antibody covalently coupled to the surface of blocked magnetic beads |
| Magnetization: |
Superparamagnetic (no magnetic memory) |
| Mean Diameter: |
1μm (nominal) |
| Density: |
2.0g/cm3 |
| Bead Concentration: |
10mg/mL |
| Binding Capacity: |
≥10μg of GST-ERK-HA (70kDa fusion protein)/mg of beads |
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| Better immunoprecipitation results with Thermo Scientific Pierce Anti-HA Magnetic Beads. Using a KingFisher Flex Instrument with 96 deep-well plates, 25μL each of Pierce Anti-HA Magnetic Beads, Anti-HA-tag Magnetic Beads (MBL International Corp., Supplier 1) and SPHERO* Rabbit Anti-HA Magnetic Beads (Spherotech Inc., Supplier 2) were used to immunoprecipitate GST-PI3K-SH2-HA from 50μg of E. coli lysate in duplicate. Captured protein was eluted with 0.1M glycine, pH 2.0, and then resolved by SDS-PAGE and analyzed by Western blot for the HA-tagged protein. |
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Significantly better binding capacity with Thermo Scientific Pierce Anti-HA Magnetic Beads. Binding capacity was determined manually using a magnetic stand. HA-tagged protein (HA-ERK-GST, 400μg in PBS) was incubated with 100μL each of Pierce Anti-HA Magnetic Beads and Anti-HA-tag Magnetic Beads from MBL International for 1 hour at room temperature. Bound HA-tagged protein was measured using the Thermo Scientific Pierce BCA Assay by subtracting the amount of protein in the flow-through from the amount loaded onto the beads. The Pierce beads bound four-fold more protein than the equivalent amount of MBL beads. |
Related Products:
Pierce Magnetic HA-Tag IP/Co-IP Kit
Other Pierce Magnetic Beads
Pierce HA-Tag IP/Co-IP Kit (agarose)
Pierce Anti-HA Agarose and Peptide
Anti-HA Antibody
KingFisher Magnetic Particle Processors
Cell Lysis Reagents
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