Remove fusion protein purification and detection tags.
Thermo Scientific Pierce Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.
Effective – HRV 3C is designed to remove purification tags from your protein of interest
High activity at 4°C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h
Flexible – dual-tagged enzyme is compatible with various columns and buffers
Kit contains protease, cleavage buffer and control protein.
Removal of purification tags from fusion proteins
Thermo Scientific Pierce HRV 3C Protease activity is comparable to protease from other suppliers. Pierce HRV 3C Protease and enzymes from other suppliers were incubated with the GST-Syk control protein for 16h at 4°C. The reaction mixtures were then resolved by SDS-PAGE. PreScission* Protease is from GE Healthcare, Turbo3C* Protease is from Accelagen and the other HRV 3C Protease is from EMD/Millipore.
Properties of Thermo Scientific Pierce HRV 3C Protease.
Human Rhinovirus 3C
Escherichia coli (E.coli)
Optimal reaction conditions†
50mM Tris-HCl, 150mM NaCl, pH 8.0
† The enzyme can also be used in a variety of other buffers