The Thermo Scientific Pierce Fast Blocking Buffer effectively and reliably blocks Western blots in just five minutes to provide low-background results and speed up traditional blotting protocols.
Pierce Fast Blocking Buffer streamlines Western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only 5 minutes. Blocking and probing steps with Fast Blocking Buffer prevents nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). These two buffers are compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes. The blocking buffer provides rapid and effective blocking in just five minutes while producing results comparable to traditional blockers for Western blots.
- Fast – use both buffers to shorten the typical Western blot development by over 2 hours
- Simple – optimized protocol makes Western blot analysis easier than ever
- Low background – provides results comparable to classic Western blotting buffers
|Effective Western blot blocking in 5 minutes. Serial dilutions of HeLa lysate (12.5μg and down) were separated by SDS-PAGE, transferred to nitrocellulose or PVDF membrane and then blocked for 5 minutes with Thermo Scientific Pierce Fast Blocking Buffer. The blots were probed with mixtures of mouse and rabbit primary antibodies, then with HRP-conjugated secondary antibodies. Wash steps were performed with the Fast Wash Buffer. Finally, blots were detected on film via Pierce ECL Western Blotting Substrate (Part No. 32106). HH3 = Histone H3; CycB = Cyclophilin B.
Review of blocking buffers for Western blotting and ELISA
Substrates for Western blotting
Membranes for Western blotting
Stripping buffers for Western blotting
Other blocking buffers