SuperBlock Blocking Buffers

Thermo Scientific SuperBlock Blocking Buffers are optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

Blocking microplates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Highlights:

• Fast – block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
• Flexible – guaranteed to be biotin-free for use with streptavidin system
• Convenience – available in multiple buffer formats (in PBS or TBS with or without Tween*-20 detergent) and package sizes (1L, 5L and dry-blend pouches)
• Low background – the non-serum protein solution yields a high signal-to-noise ratio
• Stable – store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Product Details:

Thermo Scientific SuperBlock Blocking Buffer in PBS is better than milk for sensitive detection of target proteins. HeLa cell lysate (20, 10, 5, 2.5, 1.25, 0.625 and 0.3125µg) was separated by SDS-PAGE, and transferred to nitrocellulose (Panels A-C, Part No. 88014) or PVDF (Panels D-E, Part No. 88585) membrane. Membranes were blocked for one hour using 5% non-fat milk in Tris-buffered saline with 0.05% Tween*-20 Detergent or SuperBlock Blocking Buffer in phosphate-buffered saline with 0.05% Tween-20 Detergent. Membranes were probed for the indicated targets. Blots were incubated in Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) for 5 minutes and exposed to film (Part No. 34091).

References:

1. Ikeda, K., et al. (2003). J. Biol. Chem. 278, 7725-7734.
2. Leclerc, G.J. and Barredo, J.C. (2001). Clin. Cancer Res. 7, 942-951.
3. Subbarayan, V., et al. (2001). Cancer Res. 61, 2720-276.
4. Walters, R.W., et al. (2002). Cell 100, 789-799.

Related Resources:

Protein Methods Library: Blocking Buffers for Western Blotting and ELISA
Protein Methods Library: Blocking Strategies for Immunohistochemistry

Related Products:

All Blocking Buffers

Note: Part No. 37517 and 37537 are specifically designed to be used for Western blotting with precipitating substrates. They are not intended for use with chemiluminescent substrates or microplates (ELISA).