Zeba Spin Desalting Columns and Devices, 7K MWCO

Thermo Scientific Zeba Spin Desalting Columns, Plates and Cartridges (7K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 2µL to 4mL samples.

Zeba Desalting Columns, Plates and Cartridges are polypropylene devices containing high-performance Zeba Desalting Resin, offering rapid and exceptional protein desalting with high recovery of proteins larger than 7000 daltons. Available in 5 different spin columns sizes, 96-well filter spin plates and cartridges, Zeba Desalting Devices provide trouble-free desalting and buffer exchange for sample volumes ranging from 2µL to 4mL. The easy-to-use spin column format dramatically improves results over standard drip-column methodologies and the 96-well spin plate format enables more high-throughput processing. The Zeba Desalting Cartridges are compatible with syringe processing or will attach to popular automated liquid-chromatography systems.

Highlights:

• High recovery – low-binding resin maximizes protein recovery
• Fast – no fraction screening or waiting for protein to emerge by gravity-flow
• Easy-to-use – no cumbersome column preparation or equilibration
• Flexible – available in spin columns, filter spin plates and cartridges for a range of needs
• Economical – great performance at lower cost than other commercially available cartridges

Product Details:

Zeba Spin Desalting Columns, 7K MWCO contain a proprietary high-performance desalting resin with exceptional desalting and protein-recovery characteristics compared to other commercially available resins. Even very dilute (25µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (< 1000 MW) and good recovery of proteins and other macromolecules (> 7000 MW). Zeba Spin Desalting Columns can be used to process samples from 2 to 4000µL.

The Zeba 96-well Spin Desalting Plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100µL) in 5 minutes.

The Zeba Desalting Cartridge-fittings are compatible with syringe processing or will attach to popular automated liquid-chromatography systems such as ÄKTA* or FPLC Systems without additional connectors. Each Zeba Desalting Chromatography Cartridge includes an accessory pack that readily adapts cartridges for use with Luer-Lok* Syringe Fittings or tubing. The cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses and efficiently process samples from 50 to 1500µL.

Efficient salt removal and protein recovery with Desalting Chromatography Cartridge. Bovine serum albumin (1mg) in 1M NaCl was applied to 5-ml Thermo Scientific Zeba Desalting Cartridge at a flow rate of 5ml/minute. Cartridge profile shows isocratic elution of BSA (blue) and NaCl detected by conductivity (brown). Greater than 95% of the BSA was recovered and more than 95% of the was salt removed. Results were comparable to those obtained with desalting cartridges from other suppliers. Note: see additional Performance Data for Zeba Desalting Chromatography Cartridges, including side-by-side comparison to more expensive columns from other suppliers.

Related Resources:

Desalting Products Selection Guide
Protein Methods Library – Desalting and Gel Filtration

Related Products:

Zeba Desalting Columns and Devices, 40K MWCO
Dialysis Tubing, Cassettes and Devices
Detergent Removal Resin, Columns and Plates
Pierce Protein Concentrators

Available Formats:

• Spin Columns – 75µL, 0.5mL (microcentrifuge), 2mL, 5mL, 10mL columns
• Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing (learn more about our Chromatography Cartridges)
• 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. (see Zeba 96-well Spin Plate data)