Substrates for ELISA Detection

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.

Selection Guides – Choose an ELISA substrate:
- Soluble Substrates for ELISA Selection Guide
Protein Methods Library – Learn more about ELISA:
- Overview of ELISA
- ELISA Development Guide
Related Product Categories – Other products of interest:
- Coated microplates for ELISA
- Target-specific ELISA kits


	ABTS Substrates Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.


	OPD Substrates Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.


	QuantaBlu Fluorogenic Peroxidase Substrate Soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA that allows for stopped, non-stopped and kinetic assays.


	QuantaRed Enhanced Chemifluorescent HRP Substrate Most sensitive fluorescent ELISA substrate for peroxidase (HRP) that provides sensitivity comparable to enhanced chemiluminescence (ECL).


	SuperSignal ELISA Femto Substrate HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.


	SuperSignal ELISA Pico Substrate HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.


	TMB Substrates Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.


	PNPP Substrates Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.


	ONPG Substrate Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.


	Substrate Buffers Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.