Western Blotting, ELISA and Cell Imaging

Blocking buffers, secondary antibody conjugates, and substrate detection reagents (colorimetric, chemiluminescent and fluorescent) for sensitive and consistent protein analysis by Western blotting, ELISA and immunohistochemistry (IHC).

Selection Guides – Choose a product for your immunodetection experiment:
Protein Methods Library – Learn more protein detection techniques:
Related Videos – Watch how methods are performed:
- Western Blot Video Tutorials
Related Product Categories – Other products of interest:


	Secondary Antibodies and Conjugates Polyclonal antibodies for many species of IgG and other immunoglobulins, offered in purified (unconjugated) forms and as conjugates with HRP, AP, biotin and popular fluorophores.


	Biotin-Binding Proteins and Conjugates Streptavidin, NeutrAvidin and avidin protein conjugates for biotinylated antibody probe detection in ELISA and Western blot protocols; includes peroxidase and poly-HRP conjugates and high-intensity fluorescent and infrared conjugates.


	Antibody-Binding Proteins and Conjugates Protein A, Protein G, Protein A/G, Protein L and other antibody-binding proteins available in purified form and as biotinylated or peroxidase-labeled (HRP) conjugates for secondary detection in microplate and membrane assay techniques.


	Blocking Buffers for Protein Methods Traditional protein blocking agents (BSA, casein and milk) and exclusive Pierce blocking buffers (SuperBlock, StartingBlock, Protein-Free) for Western blotting, ELISA and other immunoassay detection methods with microplates and membranes.


	Western Blot Reagents, Kits and Equipment Colorimetric, fluorescent and chemiluminescent Western blot substrates (Pierce ECL, SuperSignal); nitrocellulose and PVDF membranes; X-ray film and accessories; and antibody stripping buffers essential for immunoblotting detection procedures.


	ELISA Reagents, Microplates and Accessories Substrates, buffers, plates and various other supplies to develop and complete ELISA and EIA experiments; includes high-binding capacity coated microplates and colorimetric (TMB), chemiluminescent and fluorogenic enzyme substrates for HRP conjugates.


	Immunohistochemistry Reagents Tissue crosslinkers, cell fixatives, formaldehyde, permeabilization reagents, precipitating enzyme substrates, dyes and staining kits for immunohistochemistry (IHC) and other histochemical detection procedures.


	Nucleic Acid Labeling and Detection Products for labeling, hybridization, electrophoresis and transfer of nucleic acids for Southern blotting, Northern blotting, gel-shift assays (EMSA) and gene expression studies.