Four colors of fluorescent probes to stain whole cells for fluorescence microscopy.
Thermo Scientific Cellomics Whole Cell Stains are four colors of reactive fluorescent dyes that evenly bind to stain entire, fixed cells for cellular imaging analysis by fluorescence microscopy.
These blue, green, orange and red fluorescent stains for whole-cell labeling are intense, highly photostable and matched to the output wavelengths of common fluorescence instrumentation. Many high-content screening (HCS) methods and other cell-imaging assays based on fluorescence microscopy require labeling of entire cells alongside detection of specific targets. Whole cell stains are used to identify and count individual cells and to define the cell region in which image analysis is applied. The primary object might be a specific protein, the nucleus or other major cellular component, an organelle or whole cells.
Whole cell fluorescent stains – reactive dyes bind to cell surfaces and contents to provide complete and even visualization of fixed cells in fluorescence imaging applications
Options for multiplexing – four fluorescent color-options enable dual staining with nearly any fluorescent reporter or fluor-labeled antibody probe
Compatible – all four fluorescent dyes match emission and excitation filters and lasers used in standard fluorescence microscopy and imaging instruments
Four colors of fluorescent whole cell stains. NIH 3T3 cells stained with Thermo Scientific Whole Cell Stains (Blue, Green, Orange and Red, respectively) for 30 minutes. Cell images were acquired on the Thermo Scientific Cellomics ArrayScan HCS Reader with a 20X objective lens.
Identify cell boundaries with whole cell stains. U2OS cells in sub-confluent culture conditions stained with Thermo Scientific Whole Cell Stain Green, allowing the cell boundary (red lines) to be identified on the image acquired on the Thermo Scientific Cellomics ArrayScan HCS Reader with a 20X objective lens and analyzed using the Cellomics Morphology Explorer BioApplication.