Small-scale protein immobilization and affinity purification made easy.
The Thermo Scientific Micro Protein Coupling Kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.
The Micro Protein Coupling Kit is for immobilizing small amounts (25 to 100µg) of purified antibody and other proteins onto Thermo Scientific AminoLink Plus Coupling Resin (crosslinked 4% beaded agarose) to create a small, reusable, microcentrifuge affinity column. The coupling and affinity purification procedures are optimized for small sample volumes (200 to 300µL). Wash and elution steps are achieved rapidly and efficiently with the convenient microcentrifuge spin columns. Each kit contains sufficient reagents for 10 coupling reactions and 20 affinity purifications.
Highlights:
Optimized for small scale – ideal for coupling small amounts (25 to 250µg) of purified antibodies, proteins and other amine-containing ligands for purification of specific antibodies from crude serum
High-performance affinity resin – covalent attachment of the protein to AminoLink Plus Coupling Resin results in a leach-resistant affinity support
Efficient coupling chemistry – immobilization efficiency greater than 80% (as measured with 4 hour coupling reaction using mammalian antibodies)
Fast and easy to use – perform wash and elution steps using a microcentrifuge
Reusable – use prepared peptide resin several times with no significant loss of capacity
Product Details:
Protocol summary for the Thermo Scientific Micro Protein Coupling Kit.
A.
Lane 1: 40µg E. coli lysate
Lanes 2-4: Eluted fractions 1-3 (30µL each)
Lane 5: MW Marker (Product No. 26681)
B.
Lane 1: 1.25µg E. coli lysate
Lanes 2-4: Fraction 1 eluates (2, 4 and 6µL)
Lane 5: MW Markers (Product No. 26651).
Affinity Purification of GST-tagged BIR2 Protein. The Thermo Scientific Micro Protein Coupling Kit was used to immobilize anti-GST antibody (87µg) to AminoLink Plus Coupling Resin with 87% coupling efficiency. The the resin was then used to affinity purify GST-tagged from 200µL of E. coli lysate expressing the recombinant protein. Gel stained with coomassie dye (A) and Western blot detected with Thermo Scientific SuperSignal Substrates (B).
