ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.
- Product Number: N502
- Size: 1 L (sufficient for thirty 96-well plates)
- Storage: Shipped on cold packs. Store at 2-8°C immediately upon receipt.
- Application: Use as a blocking buffer in ELISA. The buffer is ready to use. No dilution is required.
- Instructions for use in an ELISA: Following the incubation with a coating antibody, aspirate the coating antibody from the plate and add 300 µl of ELISA Blocker Blocking Buffer to each well. Cover the plate with a plate sealer and incubate for a minimum of 1 hour at room temperature (22-25°C). Aspirate blocking buffer and proceed with the ELISA procedure or dry the plate for future use.
- Plate Drying: Plates should be dried uncovered, at room temperature, for a minimum of 1 hour. For optimal drying, plates should not be stacked and humidity should be at 30-40%. If plates are stacked, or humidity is high, the drying time should be increased. Once drying is complete, seal each plate in a foil pouch with a desiccant. When sealed with a desiccant, plates may be stored at 2-8°C for up to 6 months.
ELISA Wash Buffer (Product # N503)
TMB Substrate Solution (Product # N301)
Standard HRP-Streptavidin for ELISA (Product # N100)
Thermo Scientific Pierce ELISA Kits
Other Blocking Buffers for ELISA and Western blotting