Microplate competitive sandwich ELISA kit for cyclic AMP (cAMP).
Assay cyclic AMP (cAMP) levels in culture supernatant, cell lysate and tissue homogenate samples (treated with 0.1M HCl and acetylated or left non-acetylated) using our Thermo Scientific Cyclic AMP Competitive ELISA Kit.
This cyclic AMP competitive ELISA kit is an immunoassay for the quantitative determination of cyclic adenosine monophosphate (cAMP) in samples treated with 0.1M HCl. The assay is based on the competition between cAMP in the standard or sample and alkaline phosphatase conjugated cAMP (cAMP-AP) for a limited amount of cAMP monoclonal antibody bound to an anti-rabbit IgG precoated 96-well plate. As the concentration of cAMP in the sample increases, the amount of cAMP-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.