Cyclic AMP Competitive ELISA

Assay cyclic AMP (cAMP) levels in culture supernatant, cell lysate and tissue homogenate samples (treated with 0.1M HCl and acetylated or left non-acetylated) using our Thermo Scientific Cyclic AMP Competitive ELISA Kit.

This cyclic AMP competitive ELISA kit is an immunoassay for the quantitative determination of cyclic adenosine monophosphate (cAMP) in samples treated with 0.1M HCl. The assay is based on the competition between cAMP in the standard or sample and alkaline phosphatase conjugated cAMP (cAMP-AP) for a limited amount of cAMP monoclonal antibody bound to an anti-rabbit IgG precoated 96-well plate. As the concentration of cAMP in the sample increases, the amount of cAMP-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.

Highlights:

• Target: cyclic adenosine monophosphate (cyclic AMP, cAMP)
• Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
• Measurement: absorbance at 405nm minus absorbance at 570nm
• Assay Range: 0.78 to 200pmol/mL (non-acetylated); 0.08 to 20pmol/mL (acetylated)
• Sensitivity: 0.39pmol/mL (non-acetylated); 0.04pmol/mL (acetylated)
• Precision: dependent upon sample type, level and assay (see instructions)
• Sample Types: cell culture supernatant, cell lysate, tissue homogenate
• Total Assay Time: 3 hours (non-acetylated); 2 hours (acetylated)
• Sample Size: 100μL
• Specificity: no significant cross-reaction with AMP, ATP, cGMP, GMP, GTP, cUMP, or CTP

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, treatment reagents, buffers, standard, substrate, and stop solution.

References:

• Cyclic AMP Competitive ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Fisher BioReagents* Forskolin from Coleus forskohlii – adenylyl cyclase activator that increases cyclic AMP production in cells
• Search cAMP Antibodies (www.pierce-antibodies.com) – matched pair links coming soon