The increased Western blot sensitivity you need with speed you want.
Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.
These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate.The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.
Highlights:
Fast – picogram levels of detection in about 1 hour
Convenient – no expensive hardware or vacuum required; no clogging issues
Simple – optimized protocol makes Western blot analysis easier than ever
Economical – cost as little as $14 per blot; no expensive consumables or extra equipment
Excellent stability – kit is stable for 1 year stored at 4°C
Easy – complete kits contain all components needed to block, probe and develop a blot with your mouse or rabbit primary antibody
Product Details:
The Pierce Fast Western Blot Kits are reagent-based system that provide optimized reagents for blocking, antibody dilution and detection of Western blots. The Fast Western Blot Kit protocol is quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other popular rapid Western blotting systems.
Classic vs. fast Western protocol using Thermo Scientific SuperSignal West Pico Substrate. 293T cell lysate was diluted in reducing sample buffer and loaded to a gel. After electrophoresis, proteins were transferred to nitrocellulose and PVDF membranes using the Thermo Scientific Pierce Fast Semi-Dry Blotter for 10 minutes at 25V. Half of both membranes was developed using the classic Western protocol, and the other half was developed using the fast Western protocol. XIAP detection: The blots were probed with mouse anti-XIAP antibody (1µg/mL, BD Pharmingen), incubated in SuperSignal West Pico Substrate Working Solution for 5 minutes and exposed to film for 1 minute. TSC1 detection: The blots were probed with mouse anti-TSC1 antibody (1/1000 dilution, Cell Signaling Technology), incubated in SuperSignal West Pico Substrate Working Solution for 5 minutes and exposed to film for 5 minutes.
Comparision of other Western blotting protocols to the Thermo Scientific Pierce Fast Western System. This table provides a comparison of the steps and times required to develop Western blot, after the transfer, using the Pierce Fast Western reagent-based system and instrument-based system from Millipore Corporation.
Protocol Steps
Classical protocol
Pierce Fast Western Blot Kit
SNAP i.d.*
Blocking/Pretreatment
60 min
- -
- -
Incubation with Primary
60 min
30 min
10 min
Wash
45 min
- -
1 min
Incubation with Secondary
60 min
10 min
10 min
Wash
45 min
15 min
1 min
Total Time
270 min
55 min
22 min
Key limitations
• Preparation of fresh non-fat dry milk blocking buffer each time
• For use with mouse or rabbit primary antibodies
• Only 2 full-sized blots per cycle. Strips limited by the format of the blot holders available
• Reliable vacuum source required
The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate is a cost-effective means to obtain Western blot results quickly. This table compares the cost of reagents and required consumables used in a classical Western blot protocol, the Pierce Fast Western Blot Kit reagent-based system and Millipore SNAP i.d. system.
Western blot reagents
Classical protocol
Pierce Fast Western System
SNAP i.d.
blot holder for instrument
- -
- -
$7.33
blotting membrane (NC)
$2.48
$2.48
$2.48
blocking buffer
$0.02
- -
$1.32
wash buffer (TBST)
$1.48
- -
$1.48
secondary antibody conjugate
$0.15
- -
$0.15
detection substrate (SuperSignal West Pico)
$4.30
- -
$4.30
film (5x7)
$1.01
$1.01
$1.01
kit's price per blot
- -
$7.92
- -
Total price per blot
$9.44
$13.44
$18.07
*Prices based on 2009 Thermo Scientific Pierce Western Blot Reagents and using SuperSignal West Pico Chemiluminescent Substrate as the detection reagent for comparable levels of sensitivity.
Formulation: Multi-component kit Sufficient For: 5 mini blots probed with mouse primary antibodies Kit Contents: Fast Western Antibody Diluent, 100mL Fast Western 10X Wash Buffer, 50mL Fast Western Mouse Optimized HRP Reagent, Pico, 5mL SuperSignal West Pico Luminol/Enhancer Solution, 25mL SuperSignal West Stable Peroxide Solution, 25mL
200mL kit
$106.00
35060
Fast Western Kit, SuperSignal West Pico, Mouse
Formulation: Multi-component kit Sufficient For: 20 mini blots probed with mouse primary antibodies Kit Contents: Fast Western Antibody Diluent, 400mL Fast Western 10X Wash Buffer, 200mL Fast Western Mouse Optimized HRP Reagent, Pico, 20mL SuperSignal West Pico Luminol/Enhancer Solution, 100mL SuperSignal West Stable Peroxide Solution, 100mL
800mL kit
$216.00
35066
Fast Western Kit, SuperSignal West Pico, Rabbit
Formulation: Multi-component kit Sufficient For: 5 mini blots probed with rabbit primary antibodies Kit Contents: Fast Western Antibody Diluent, 100mL Fast Western 10X Wash Buffer, 50mL Fast Western Rabbit Optimized HRP Reagent, Pico, 5mL SuperSignal West Pico Luminol/Enhancer Solution, 25mL SuperSignal West Stable Peroxide Solution, 25mL
200mL kit
$106.00
35061
Fast Western Kit, SuperSignal West Pico, Rabbit
Formulation: Multi-component kit Sufficient For: 20 mini blots probed with rabbit primary antibodies Kit Contents: Fast Western Antibody Diluent, 400mL Fast Western 10X Wash Buffer, 200mL Fast Western Rabbit Optimized HRP Reagent, Pico, 20mL SuperSignal West Pico Luminol/Enhancer Solution, 100mL SuperSignal West Stable Peroxide Solution, 100mL
