Microplate kits and reagent sets for sandwich ELISA of human epidermal growth factor (EGF).
Assay EGF (epidermal growth factor) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human EGF ELISA Kits.
These human EGF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of epidermal growth factor (EGF, also called URG, HOMG4) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched EGF antibody pairs and EGF standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant human epidermal growth factor (EGF); also known as urogastrone (URG), hypomagnesemia type 4 (HOMG4)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with human HB-EGF (Heparin-binding EGF), mouse EGF, rat EGF and human IL-2, IL-4, IL-6, IL-8, TNFα TGFβ, GM-CSF
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).
Human EGF ELISA standard curve. An example standard curve for the Thermo Scientific Human EGF ELISA Kit (Part No. EHIFNG), with error bars based on two replicates for each epidermal growth factor standard concentration. Also see standard curves for the EGF Reagent Kit and EGF Antibody Pair Kit.