Thermo Scientific Pierce Competition RED Device Inserts and Base Plates comprise a specially designed system for evaluating competitive binding interactions using rapid equilibrium dialysis (RED).
The Competition RED Device was developed in association with pharmaceutical laboratories to more accurately model in vivo drug interactions by enabling equilibrium dialysis experiments to be performed in a multiplexed format involving competition for binding among different tissues. The Competition RED Device System consists of disposable dialysis tube inserts and a reusable Base Plate made of high-grade PTFE. The Base Plate is much like our regular RED Device Plate except that it is divided into different size chambers (wells) for positioning 2-8 RED Device Inserts per well. The format enables competitive dialysis experiments involving 2-15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes a selection of both types). The Competition RED Device requires no extensive assembly or specialized equipment, and each chamber/well is easily accessible from the top of the device.
Highlights:
- Easy to use – disposable tubes require no presoaking step, assembly, or specialized equipment
- Short incubation time – design provides high surface-to-volume ratio of membrane to sample, enabling equilibrium to be reached within 2-4 hours
- Flexible format – base plate contains several different chamber sizes, enabling small molecule partitioning studies involving 2-16 tissue or protein samples without waste
- Robust – compartmentalized design eliminates potential for cross talk or leakage
- Reproducible and accurate – perform controlled experiments with multiple tissues to obtain screening results that have high predictability for in vivo studies with animal models
- Validated quality – base plate is composed of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination; each lot is functionally tested in a protein binding assay for guaranteed performance
Applications:
- ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
- Aids in determining formulation of drug dosage for in vivo studies
- Drug-drug interaction studies
- Competitive binding and dissociation constant determination for small molecules versus multiple targets
Product Details:
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| Format of the Thermo Scientific Competition RED Device Base Plate. The Base Plate body (center) has different well sizes for holding 4, 6, 8 or 16 dialysis chambers (2, 3, 4 or 8 inserts, respectively). The different wells allow a choice of the size best suited for the specific experimental design. The lid (left) snaps onto the Base Plate and holds and suspends the dialysis chambers in the wells.The device requires no extensive assembly steps or specialized equipment, and each chamber/well is easily accessible from the top of the device. The base plate has a standard 96-well plate footprint with 9 x 9 mm well spacing. The Competition RED Device Inserts (pictured above) are offered with the molecular weight cutoff (MWCO) of 12,000Da. |
| Sample volume required for each Base Plate well size. |
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Base Plate Well
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Number of Inserts
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Total Volume
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4-compartment
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2 inserts
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1.5mL
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6-compartment
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3 inserts
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2.5mL
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8-compartment
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4 inserts
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3.0mL
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16-compartment
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8 inserts
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5.0mL
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| Note: add 0.5mL for each insert slot that is not filled |
Determining the extent to which a molecule partitions between plasma and specific tissues in the body is a critical phase of drug development because it determines compound dosing, efficacy, clearance rate, and the potential for drug interactions or tissue damage. Although final assessment of these variables invariably requires animal dosing and analysis, in vitro screening systems that are rapid and have good predictability are highly desirable for ADME-Tox studies. Equilibrium dialysis is among the most successful of in vitro screening method for evaluating drug binding and dosage requirements. Equilibrium dialysis is used to estimate the non-bound drug fraction in plasma or drug partitioning between red blood cell and plasma, plasma and liver microsomes, or plasma and tissue homogenates. However, many tissue-partitioning studies indicate that certain drugs show significant binding to tissues tested individually but have considerably different binding preferences when placed in competition between multiple tissues. The Competition RED Device extends the power of equilibrium dialysis by facilitating analysis of simultaneous drug interactions and partitioning among multiple tissues.
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Example device setup and experiment. This setup uses a 6-compartment well of the base plate, two dual-chamber inserts (top and bottom) and one single-chamber insert (middle position). Each 10-pack of Competition RED Inserts contains eight dual-chamber inserts and two single-chamber inserts. The open chamber in the single-chamber inserts enables direct access to the sample in the base plate well without disassembling the device. This example experiment monitors drug partitioning among buffer, plasma, heart, liver, kidney and brain tissue extracts. The buffer chamber is for determining the amount of unbound drug.
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Posters: Download a PDF version of the following posters.
In-vitro Tissue Protein Binding in rat, dog, monkey and human (Opens new broswer window)
Cindy Xia¹, Mark Qian¹, Susan Chen¹, Frank Lee¹, Tai-Nang Huang²,
¹Drug Metabolism and Pharmacokinetics, Millennium Pharmaceuticals, The Takeda Oncology Company, Cambridge, MA.
²DMPK, Linden Bioscience, Research Triangle Park, North Carolina
Characterization of Drug Distribution in Tissues Using a Rapid Equilibrium Dialysis Device (Opens new broswer window)
Mark G. Qian, Susan Chen, Debra Liao, Tai-Nang Huang*, Jing-Tao Wu, and Frank Lee DMPK,
DMPK/Drug Safety & Disposition, Millennium Pharmaceuticals, Inc., Cambridge, MA 02139, USA
*Linden Bioscience, Lexington, MA, USA
Species Relative Plasma Protein Binding (Opens new broswer window)
Ken Matuszak and Corey Custer,
Drug Metabolism and Disposition, Pharmacokinetics and Bioanalysis, Abbott Laboratories, Abbott Park, IL, USA
Related Products:
Other Rapid Equilibrium Dialysis (RED) Products and Accessories
RED Device Inserts (single chamber)
Pierce Protein Precipitation Plates
Sealing Tape for Microplates
Phosphate Buffered Saline
LC/MS Grade Acetonitrile
Trifluoroacetic Acid
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