Measure activated (cleaved) poly (ADP-ribose) polymerase (PARP) in whole cells.
Thermo Scientific Pierce Cleaved PARP In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure activated (cleaved) poly (ADP-ribose) polymerase (PARP) and tubulin in whole cells.
These ELISA kits for whole cells include a rabbit monoclonal anti-(cleaved) PARP antibody, mouse monoclonal anti-alpha-Tubulin antibody, detection reagents and all buffers required for either colorimetric or near infrared detection. The Cleaved PARP In-Cell ELISA Kits enable simultaneous detection of both Cleaved PARP and Tubulin as a reference.
Highlights:
Measures activated (cleaved) poly (ADP-ribose) polymerase (PARP) and tubulin in whole cells using 96-well or 384-well microplates
colorimetric detection kit for use with standard microplate readers
near infrared fluorescent detection kit for use with IR imaging system
Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
Provides better data and higher throughput than quantitative Western blotting
Less costly (one well vs. one lane) than Western blotting
Overview of PARP:
Poly (ADP-ribose) polymerase (PARP) plays a role in repair of single stranded DNA nicks. PARP also regulates proteins such as histones and telomerase through ADP-ribosylation and is an important marker of caspase 3-mediated apoptosis. In response to apoptotic signals, activated caspase 3 cleaves the 116 kDa PARP protein into 85 kDa and 25 kDa fragments. In humans, PARP cleavage occurs at Asp 214 and Gly 215 residues. Cleavage of PARP correlates with DNA fragmentation and other morphological changes making it a critical marker for apoptosis. The Cleaved PARP In-Cell ELISA Kit enables simultaneous detection of both Cleaved PARP and Tubulin as a reference.
