Thermo Scientific Pierce High Sensitivity NeutrAvidin Protein Coated Plates are biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte.
These Thermo Scientific Pierce Coated Plates are coated using proprietary surface-activation technology to achieve maximum efficient coating of active streptavidin biotin-binding protein. The high sensitivity plates enable detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins. Whether to detect signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.
Highlights:
- Sensitive – detect down to 5ng/mL of biotinylated IgG
- Specific – low background with high signal-to-noise ratios
- Versatile – use either fluorescence, chemiluminescence or colorimetric detection
- Robust – broad dynamic range
- Ready to use – plates are supplied pre-blocked to save time
Product Details:
Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.
Comparison of NeutrAvidin Coated Plate types. Detection Ranges were determined using Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate.
| |
High Sensitivity
(HS) |
High Binding
Capacity (HBC) |
Standard Binding
Capacity (SBC) |
| Application |
Detect low concentrations of biotinylated molecules |
Detect high concentrations of biotinylated molecules |
General ELISA screening applications |
| Biotinylated Protein Minimum Size |
> 26kDa |
> 8kDa |
> 8kDa |
Detection Range
NeutrAvidin Plates |
5 to 125ng/mL
(current page) |
15 to 2500ng/mL
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15 to 300ng/mL
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Detection Range
Streptavidin Plates |
5 to 300ng/mL
(visit page) |
62 to 10,000ng/mL
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31 to 1250ng/mL
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Related Resources:
Review of avidin-biotin systems
Review of ELISA methods
Related Products:
Other Streptavidin and NeutrAvidin Coated Microplates
Blocking Buffers
ELISA Substrates |
