High-capacity binding of biotinylated antibodies and other molecules.
Thermo Scientific High-Binding Capacity NeutrAvidin Protein (HBC) Coated Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.
The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.
Unique plate-coating technology – results in high loading and binding capacity
NeutrAvidin Protein option – coated plates have up to twice the binding capacity of typical plates
Broader dynamic range – extends the quantitative range so there is less need for dilutions
Save time – pre-blocked plates to reduce the number of assay steps
Flexible assay formats – coated plates offered in 8-well strips to reduce waste
Comparison of Thermo Scientific NeutrAvidin High Binding Capacity (HBC) Coated Plate, NeutrAvidin Regular Binding Capacity (RBC) Coated Plates and a competing Streptavidin Coated High Binding Capacity Plate (CHC). Plates were incubated with various dilutions of biotinylated, phosphorylated peptide. After washing, the plates were incubated with mouse anti-phosphotyrosine antibody and then detected using an anti-mouse-FITC conjugate.
Plate Characteristics for High Binding Capacity Biotin-Binding Plates.