High-capacity binding of biotinylated antibodies and other molecules.
Thermo Scientific High-Binding Capacity NeutrAvidin Protein (HBC) Coated Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.
The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.
Highlights:
Unique plate-coating technology – results in high loading and binding capacity
NeutrAvidin Protein option – coated plates have up to twice the binding capacity of typical plates
Broader dynamic range – extends the quantitative range so there is less need for dilutions
Save time – pre-blocked plates to reduce the number of assay steps
Flexible assay formats – coated plates offered in 8-well strips to reduce waste
Product Details:
Comparison of Thermo Scientific NeutrAvidin High Binding Capacity (HBC) Coated Plate, NeutrAvidin Regular Binding Capacity (RBC) Coated Plates and a competing Streptavidin Coated High Binding Capacity Plate (CHC). Plates were incubated with various dilutions of biotinylated, phosphorylated peptide. After washing, the plates were incubated with mouse anti-phosphotyrosine antibody and then detected using an anti-mouse-FITC conjugate.
Plate Characteristics for High Binding Capacity Biotin-Binding Plates.
