Microplate kits and reagent sets for sandwich ELISA of human interferon gamma (IFN-gamma).
Assay IFN-γ (human interferon gamma) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Human IFN gamma ELISA Kits.
These human IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Highlights: †
Target: natural and recombinant human interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, TNFα or GM-CSF
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.
Includes:
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).
Human IFNγ ELISA standard curve. An example standard curve for the Thermo Scientific Human IFNγ ELISA Kit (Part No. EHIFNG), with error bars based on six replicates for each interferon gamma standard concentration. Also see standard curves for the IFNγ Reagent Kit and IFNγ Antibody Pair Kit.
Formulation: Complete ELISA kit with coated 96-well plates Sufficient For: 2 x 96 microplate wells Kit Contents: Anti-Human IFN gamma Precoated 96-well Strip Plate, 2 each Lyophilized Recombinant Human IFN gamma Standard, 4 vials Standard Diluent, 2 x 12mL Biotinylated Antibody Reagent, 2 x 8mL 30X Wash Buffer, 2 x 50mL Streptavidin-HRP Concentrate, 2 x 75μL HRP Dilution Buffer, 2 x 14mL TMB Substrate Solution, 2 x 13mL Stop Solution, 2 x 13mL Adhesive Plate Covers, 12 each
2-plate kit
$600.00
EHIFNG5
Human IFN gamma ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates Sufficient For: 5 x 96 microplate wells Kit Contents: Anti-Human IFN gamma Precoated 96-well Strip Plate, 5 each Lyophilized Recombinant Human IFN gamma Standard, 5 vials Standard Diluent, 75mL Biotinylated Antibody Reagent, 35mL 30X Wash Buffer, 200mL Streptavidin-HRP Concentrate, 250μL HRP Dilution Buffer, 70mL TMB Substrate Solution, 5 x 13mL Stop Solution, 55mL Adhesive Plate Covers, 30 each
5-plate kit
$1,779.00
ESS0002
Human IFN gamma ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA Sufficient For: 5 x 96 microplate wells Kit Contents: IFN gamma Coating Antibody, 1 vial IFN gamma Detection Antibody, 1 vial Human IFN gamma Standard, 5 vials Streptavidin-HRP Conjugate, 0.25mL TMB Substrate Solution, 55mL Stop Solution, 55mL
5-plate kit
$372.00
KHIFNG
Human IFN gamma ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA Sufficient For: 40 x 96 microplate wells Kit Contents: IFN gamma Coating Antibody, 1 vial IFN gamma Biotinylated Detection Antibody, 1 vial Human IFN gamma Standard, 1 vial
40-plate kit
$999.00
SIFNG
Human IFN gamma Standard for ELISA
Formulation: Recombinant human IFN gamma from E. coli; Lyophilized powder; contains BSA; Calibrated to NIAID reference lot Gxg-01-902-535 Sufficient For: ELISA standard curve with replicates
