Creates 11-angstrom crosslinks between amines while maintaining overall charge properties.
Thermo Scientific Pierce DMS is dimethyl suberimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of an 8-atom spacer arm.
Highlights
Water-soluble
Rapid reaction with amines at alkaline pH values (pH 8-10)
Amidine bond retains net charge character of protein
Reversible at high pH values
Reactive groups: imidoester (homobifunctional)
Reactive toward: amino groups
Product Details:
References
Dihazi, G.H. and Sinz, A. (2003). Rapid Commun. Mass Spectrom. 17, 2005-2014.
Wang, D. and Moore, S. (1977). Polyspermine-ribonuclease prepared by crosslinkage with dimethyl suberimidate. Biochem. 16(13), 2937-2941.
Formulation: Dimethyl suberimidate-2HCl; powder-granule solid Sufficient For: 3660mL of typical 1X (1mM) reaction solution, or approx. 500 uses at 2mg reagent per use
