Thermo Scientific Pierce C18 Tips are ready-to-use pipette-tip columns of C18 resin that enable fast and efficient capture, concentration, desalting and elution of peptides for MALDI mass spectrometry and other methods.
Improve protein analysis results with Pierce C18 Tips by removing urea, salts and other contaminants before MS analysis. The tips are ideal for preparing samples for matrix-assisted laser desorption ionization (MALDI) or nanoelectrospray ionization techniques. The Pierce C18 Tips are available in convenient 10 and 100µL tips with binding capacities of 8 and 80µg, respectively.
- Monolithic C18 fast-flow tips have low resistance and improved flow characteristics compared to other commercially available tips
- High binding capacity in a convenient tip format
- Two pipette tip formats for processing up to 8 or 80µg of sample
- Eight packs and 96-tip trays are automation compatible
These C18 tips offer excellent flow properties with a high-efficiency monolithic C18 sorbent for fast wetting, loading, washing and eluting. The five-step procedure is simple and requires less than 5 minutes to process protein digests, strong cation exchange fractions and other protein and peptide samples for mass spectrometric (MS) analyses.
|Removing urea and salt eliminates interference in MS chromatograms. A bovine serum albumin (BSA) tryptic digest was analyzed on a Thermo Scientific MALDI-Orbitrap XL Hybrid Mass Spectrometer. Digests and samples containing 150mM NaCl and 4M urea were analyzed with or without processing with Pierce C18 Tips (10µL).
|Thermo Scientific Pierce C18 Tips outperform tips from other suppliers. BSA tryptic digests were analyzed either directly on a Thermo Scientific LTQ-XL Ion Trap Mass Spectrometer or after processing with Pierce C18 Tips (100µL) or supplier X tips. Base peak chromatograms of the peptide elution were extracted from the data sets to evaluate sample complexity and chromatographic resolution. MS results were analyzed with Matrix Science Mascot and the SwissProt Release 52 database to determine protein sequence coverage.
Review of sample preparation for mass spectrometry
Mass Spectrometry-Grade Endoproteinases
Pierce C18 Spin Columns