|
 Thermo Scientific HeavyPeptide AQUA Custom Synthesis Service provides isotopically labelled, AQUA-grade peptides for the relative and absolute quantitation of proteins at very low concentrations in complex protein mixtures.
HeavyPeptide sequences up to 15 amino acids in length are synthesized using the latest Fmoc solid-phase technology and purified by HPLC. All HeavyPeptide sequences come with mass spectrometric analysis, and AQUA (Absolute QUAntitation) Ultimate and QuantPro-grade peptides also come with stringent analytical HPLC to determine the final purity and assure that you are receiving only the highest quality peptides for absolute quantitation. We offer advanced heavy peptide synthesis capabilities with a wide range of labels, modifications, scales and purities to meet your research needs. HeavyPeptide AQUA-grade peptides are also part of our HeavyPeptides FasTrack Service to accelerate targeted assay development.
These peptides are packaged using our ArgonGuard service, in which peptides are packaged in argon gas to minimize amino acid oxidation during shipping and storage. This standard service helps maintain biological activity of custom peptides and reduce experimental variation.
Highlights:
- Accurate – concentration precision to meet your application needs
- Consistent results – ArgonGuard service helps maintain biological activity during shipping and storage
- Sensitive – enables the absolute quantification of low-abundant proteins
- Specific – 100% peptide sequence specificity
- Flexible – extensive list of available modifications, solvents, concentrations, aliquots and formats
Includes:
- One isotopically labelled peptide (HeavyPeptide) or non-labelled control peptide (LightPeptide) sequence.
Applications:
- Biomarker discovery, verification and validation
- Functional quantitative proteomics
- Quantitation of post-translationally modified proteins
- Confirmation of results from RNA interference (RNAi)
- Pharmacokinetics
- Metabolomics
- Clinical biochemistry for drug and metabolite monitoring
- Anti-doping testing
- Protein expression monitoring
- Cell signal profiling and pathway validation
Product Details:
|
Thermo Scientific HeavyPeptide and LightPeptide AQUA Grades
|
| Grade |
Description |
|
AQUA Ultimate |
Fully solubilized peptides with a precision equal or better than ±5% that are the best choice for biomarker validation and experiments demanding ultimate quantitative precision and batch-to-batch reproducibility. |
|
AQUA QuantPro
|
Fully solubilized peptides with a concentration precision equal or better than ±25% that are ideal for biomarker verification. |
| AQUA Basic |
Lyophilized peptides that are more adequate for relative quantitation. The batch-to-batch consistency is difficult to predict. |
|
Specifications of Thermo Scientific HeavyPeptide and LightPeptide AQUA-Grade Standards
|
| Grade |
AQUA Ultimate |
AQUA QuantPro |
AQUA Basic |
|
Formulation |
5pmol/μL in 5% (v/v) acetonitrile/H2O |
5pmol/μL in 5% (v/v) acetonitrile/H2O |
Lyophilized |
|
Actual concentration
|
Measured by AAA† |
Measured by AAA† |
Measured by AAA† |
| Concentration precision |
±5% |
±25% |
|
|
Peptide purity
|
>97% |
>97% |
>95% |
| Isotopic enrichment |
>99% |
>99% |
>99% |
| Peptide length |
Up to 15 amino acids |
Up to 15 amino acids |
Up to 15 amino acids |
| Amount/No. of aliquots |
10nmol/10 aliquots
40nmol/40 aliquots
96nmol/96 aliquots |
10nmol/10 aliquots
40nmol/40 aliquots
96nmol/96 aliquots |
15 to 30 nmol†† (0.05 to 0.1mg)/1 aliquot |
| Quality control |
MALDI MS and analytical HPLC |
MALDI MS and analytical HPLC |
MALDI MS and analytical HPLC |
| Delivery time§ |
4 weeks |
4 weeks |
4 weeks |
| Shipment |
in solution on wet ice |
in solution on wet ice |
dry at room temp. |
| Product options |
- Additional light amino acids to extend the peptide length
- Additional heavy amino acid on each peptide
- Multiple solvents, concentrations and aliquot sizes available
- Peptides delivered in various formats (i.e., 96-well plate with or without detachable tubes in glass or plastic, 2D barcodes, etc.)
|
| Peptide modifications |
- Single or double phosphorylation (pY, pT or pS)
- Cysteine carbamidomethylation (CAM)‡
- Chloro-L-Tyrosine
- Pryoglutamic acid
- Methionine oxidation (Met[O])
- Other modifications available on request
|
†Amino acid analysis
§These production times are estimates that vary based on the number of kits ordered.
††The 30nmol amount is valid for peptides 6-15 amino acids in length. For shorter or longer peptides, the amount might decrease to as little as 15nmol.
‡ CAM tends to cause cyclisation at the N-terminus. Fully cyclized form can be provided upon request.
|
|
Heavy amino acids offered with Thermo Scientific HeavyPeptide Custom Synthesis†
|
| Amino acid |
Code |
Mass difference |
Isotope |
Isotopic enrichment |
|
Alanine |
A |
+4Da |
U-13C3, 15N |
>99% |
| Arginine |
R |
+10Da |
U-13C6, 15N4 |
>99% |
| Isoleucine |
I |
+7Da |
U-13C6, 15N |
>99% |
|
Leucine |
L |
+7Da |
U-13C6, 15N4 |
>99% |
| Lysine |
K |
+8Da |
U-13C6, 15N2 |
>99% |
| Phenylalanine |
F |
+10Da |
U-13C9, 15N |
>99% |
| Proline |
P |
+6Da |
U-13C5, 15N |
>99% |
| Valine |
V |
+6Da |
U-13C5, 15N |
>99% |
| † Other amino acids on request. |
Every peptide synthesis is a challenge. Over the last 12 years, our team has accumulated tremendous expertise through tens of thousands of successful custom peptides syntheses. By carefully listening to our world-wide customer base, we are constantly adapting our product offering to your needs. For example, your custom peptides are now packaged using our ArgonGuard packaging service to maximize biological activity during shipment and storage. The noble gas argon is added to the vial during peptide packaging, which minimizes potential oxidation of peptides containing cysteine, methionine or tryphtophan (1).
Our team of expert peptide scientists will support you starting from the design phase of your peptide to the right modifications, scale or purity for your assay to help you obtain the best experimental results for your application.
References:
- McKinnie S. M. K. et al. (2012) The solid phase supported peptide synthesis of analogues of the lantibiotic lactocin s. MedChemComm.
- Lehmann E. et al. (1999) Matrix-assisted laser desorption/ionization mass spectra reflect solution-phase zinc finger peptide complexation. J Am Soc Mass Spectrom. 10, 27-34.
- Yu Y. et al. (2009) A site-specific, multiplexed kinase activity assay using stable-isotope dilution and high-resolution mass spectrometry. Proc Natl Acad Sci U S A. 106, 11606-11.
- Wepf A. et al. (2009) Quantitative interaction proteomics using mass spectrometry. Nat Methods. 6, 203-5.
- Prakash A. et al. (2009) Expediting the development of targeted SRM assays: Using data from shotgun proteomics to automate method development. J Proteome Res. 8, 2733-9.
- Anderson N. L. et al. (2009) A human proteome detection and quantitation project. Mol Cell Proteomics. 8, 883-6.
- Lange V. et al. (2008) Selected reaction monitoring for quantitative proteomics: A tutorial. Mol Syst Biol. 4, 222.
Related Resources:
Peptide Analysis Tool
Custom Peptide Synthesis Service FAQ
Protein Methods Library: Peptide Synthesis
Protein Methods Library: Peptide Design
Protein Methods Library: Quantitative Proteomics
Related Products:
HeavyPeptide AQUA FasTrack Service
HeavyPeptide IGNIS Prime Peptide Quantitation Kit
Custom Peptide Synthesis Service
PEPotec SRM Custom Peptide Libraries
PEPotec Immuno Custom Peptide Libraries
Peptide Sample Prep Tools for Mass Spectrometry
1-Step Heavy Protein In Vitro Protein Expression Kits
|
Thermo Scientific HeavyPeptide Custom Synthesis Quote Request and Ordering Information
|
To obtain a quote:
- Download the Quote Request/Order Form (1.4mb)
- Complete all contact and billing information
- List peptides, modifications and optional services
|
|
For North America
Email quote requests or orders to
peps@thermofisher.com
OR
Contact your local sales representative or call 1-800-874-3723 or 1-815-968-0747.
|
For Europe and all other regions
Email quote requests to services.biopolymers@thermofisher.com
Email orders to sales.peptides@thermo.com
Or contact your local sales representative or Thermo Fisher Scientific in Ulm, Germany at +49-731-29870. |
For technical support, please call
1-800-874-3723 or 1-815-968-0747 |
For technical support, please call +49-731-29870. |
|
