Thermo Scientific Pierce Strong Cation and Anion Exchange Spin Columns enable rapid protein fractionation and sample preparation based on charge differences.
Pierce Strong Ion Exchange Spin Columns use membrane-adsorption as a chromatographic method to fractionate proteins based on their charge differences. The matrix has a highly porous structure with pores larger than 3000nm, providing proteins easy access to the membrane’s charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.
Highlights:
- Fast and simple – membrane-based spin format eliminates column packing
- Convenient and expandable – centrifugal format enables convenient processing of multiple samples in parallel
- Robust – membrane adsorber spin columns do not crack or run dry
- Low bed volume – small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions
Available Formats:
- Mini Format: for binding up to 4mg (500µL capacity)
- Maxi Format: for binding up to 80mg (20mL capacity)
Applications:
- Pre-fractionation of proteins in lysate
- Scouting purification conditions for new protein preparation protocols
- Removal of endotoxins from monoclonal antibodies
- Polishing His-tagged proteins after metal chelate chromatography
- Purification and concentration of proteins
- Purification of antibodies from serum, ascites or tissue culture supernatant
- Removal of detergent from protein solutions
- Sample preparation before 1D or 2D PAGE
- Purification of phosphopeptides before MS analysis
Product Details:
 |
| Protocol summary for Thermo Scientific Pierce Strong Cation and Anion Ion Exchange Spin Columns. |
Ion exchange chromatography separates molecules based on differences in their accessible surface charges. This technique is widely used in the pre-fractionation or purification of a target protein from crude biological samples. Novel membrane-based ion exchange chromatography is attracting attention because of its advantages over resin-based column chromatography. Membrane-based ion exchange chromatography has shorter diffusion times than resin-based chromatography. Interactions between molecules and active sites on the membrane occur in a convective manner through pores, which shortens the diffusion time compared with fluid inside the pores of a resin particle. Also, the relatively mild binding and eluting conditions of this separation method produce high protein recovery with intact biological activity.
Related Products:
Fe-NTA Phosphopeptide Enrichment Kit
C18 Spin columns
Cell Lysis Reagents for Protein Extraction
Subcellular Fractionation Kits
|
