The alternative to radioisotopic detection with Northern or Southern blots.
The Thermo Scientific Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications.
Nearly any DNA or RNA method based on nitrocellulose or nylon membranes and biotinylated probes can be detected with sensitivity equivalent to traditional radioactive (32P) systems. The module consists of the essential detection components used in the Thermo Scientific North2South Kit and Thermo Scientific LightShift EMSA Kit. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that provide for fast, trouble-free detection on film or by CCD camera. Obtain publication-quality blots having excellent signal and low background.
Highlights:
Fast and sensitive – achieve the same speed and detection levels you’ve come to expect with Thermo Scientific North2South and LightShift Kits
Versatile – kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
Flexible – kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed
Northern blot analysis of mouse total RNA using the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module. Total RNA samples (1µg) prepared from the indicated tissues were electrophoresed on a denaturing 1% agarose gel, transferred to Biodyne B Positive Nylon, UV crosslinked and hybridized with a biotinylated c-myc/exon 3 RNA probe (4ng/mL). Exposure time to film was 1 minute.
References:
Matata, B. M. and Galiñanes, M. (2002). J. Biol Chem.277(3), 2330–2335.
Oltra, E., et al. (2003). Endocrinology144(7), 3148-3158.
