DyLight 594

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 594 generates red fluorescence and shows wide excitation wavelength separation from green fluorophores for multi-color applications with reduced bleedthrough. The physical characteristics of DyLight 594 are comparable to other 594 dyes, is stable over a wide pH range and exhibits higher solutility than traditional fluorescent dyes. DyLight 594 is available as reactive labeling agents and as conjugates of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

Page Contents:

• DyLight 594 Properties and Applications
• DyLight 594 Products
• DyLight 594 References

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DyLight 594 Properties and Applications

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DyLight 594 Products

DyLight 594 Reactive Dyes and Kits:

• Amine-reactive dye (N-hydroxysuccinimide (NHS) ester-activated)
• Sulfhydryl-reactive dye (maleimide-activated)

DyLight 594-conjugated Fluorescent Probes:

• Secondary antibody-conjugates – Goat Anti-Mouse or Anti-Rabbit
• Streptavidin-conjugates
• Neutravidin protein-conjugates

DyLight 594 References

1. Chen Y. et al. (2010) Hepatic differentiation of liver derived progenitor cells and their characterization by microrna analysis. Liver Transplantation. 16, 1086-97.
2. Ciruela F. et al. (2010) Evidence for oligomerization between gabab receptors and girk channels containing the girk1 and girk3 subunits. Eur J Neurosci. 32, 1265-77.
3. Dimitrov E. and Usdin T. B. (2010) Tuberoinfundibular peptide of 39 residues modulates the mouse hypothalamic-pituitary-adrenal axis via paraventricular glutamatergic neurons. J Comp Neurol. 518, 4375-94.
4. Ferrati S. et al. (2010) Intracellular trafficking of silicon particles and logic-embedded vectors. Nanoscale. 2, 1512-20.
5. Haces M. et al. (2010) Selective vulnerability of brain regions to oxidative stress in a non-coma model of insulin-induced hypoglycemia. Neuroscience. 165, 28-38.
6. Huang J. et al. (2009) Retinoic acid signalling induces the differentiation of mouse fetal liver derived hepatic progenitor cells. Liver International. 29, 1569-81.
7. Lei X. et al. (2010) The 3c protein of enterovirus 71 inhibits retinoid acid-inducible gene i-mediated interferon regulatory factor 3 activation and type i interferon responses. Journal of Virology. 84, 8051.
8. Ouellet E. et al. (2010) Parallel microfluidic surface plasmon resonance imaging arrays. Lab on a Chip. 10, 581-8.
9. Rose E. et al. (2009) Glutamate transporter coupling to na, k-atpase. Journal of Neuroscience. 29, 8143.
10. Sarkar P. et al. (2010) Photophysical properties of a new dylight 594 dye. Journal of Photochemistry and Photobiology B: Biology. 98, 35-9.
11. Serda R. et al. (2009) Quantitative mechanics of endothelial phagocytosis of silicon microparticles. Cytometry Part A. 75, 752-60.
12. Singh S. K. et al. (2010) Tumour-associated glycan modifications of antigen enhance mgl2 dependent uptake and mhc class i restricted cd8 t cell responses. Int J Cancer.
13. Veenstra J. (2009) Peptidergic paracrine and endocrine cells in the midgut of the fruit fly maggot. Cell and Tissue Research. 336, 309-23.
14. Wei L. et al. (2009) Domain-and species-specific monoclonal antibodies recognize the von willebrand factor-c domain of ccn5. Journal of Cell Communication and Signaling. 3, 65-77.
15. Wei L. et al. (2010) A monoclonal antibody approach to ccn5 domain analysis. CCN proteins in health and disease, 77-95.
16. Zhu G. et al. (2009) Activation of rxr and rar signaling promotes myogenic differentiation of myoblastic c2c12 cells. Differentiation. 78, 195–204.

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