Microplate kits and reagent sets for sandwich ELISA of interleukin-17 (IL-17A, CTLA8).
Assay IL-17A (human interleukin-17A) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human IL-17A ELISA Kits.
These human IL-17A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-17 (IL-17A, CTLA8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant human interleukin-17A (also called CTLA8)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF
† Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
Human IL-17A ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-17A ELISA Kit (Part No. EHIL17A), with error bars based on twelve replicates for each IL-17 standard concentration. Also see standard curve for the IL-17A Reagent Kit.