A highly active endonuclease that degrades all forms of DNA and RNA.
Thermo Scientific Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has high specific activity (100-fold greater than DNase I) and increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99 pure enzyme, is free of any measurable protease activity and is supplied at 250U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase* Nuclease (EMD Merck).
Highlights:
Broad spectrum – degrades all forms of DNA and RNA
Highest-quality enzyme – nuclease is ≥99% pure, as tested by SDS-PAGE
Robust activity – 100-fold greater specific activity than DNase I
Versatile – can be used with a wide variety of cell lysis reagents
Applications:
Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts
Remove DNA and RNA from recombinant protein preparations prior to downstream processing
Product Details:
Pierce Universal Nuclease for Cell Lysis is commonly used to reduce the viscosity of bacterial and mammalian protein extracts for downstream application by removing the nucleic acids from protein preparations. The enzyme completely digests nucleic acids to oligonucleotides that are less than 5 bases long. Pierce Universal Nuclease for Cell Lysis helps to improve the separation of the lysate pellet from the supernatant, enhances filtration of the treated lysate, improves chromatography processing time and increases the overall protein yield. The endonuclease has also been shown to improve the compatibility of protein extracts for 2D gel electrophoresis. One unit corresponds to the amount of enzyme required to produce a change of 1.0 in the absorbance at 260nm of sonicated Herring DNA over 30 minutes at 37°C, as determined using standard nuclease from the Merck* Serratia marcescens volumetric activity assay.
Pierce Universal Nuclease activity. Cells where suspended in B-PER (A) or B-PER with lysozyme (B) with increasing concentrations of Pierce Universal Nuclease for Cell Lysis and incubated at room temperature for 30min. The lysates were then cleared by centrifugation and resolved on a 1% agarose gel, and nucleic acids were stained with ethidium bromide and visualized under ultraviolet (UV) light. M, DNA ladder. Cells that were lysed with B-PER with lysozyme but without Pierce Universal Nuclease for Cell Lysis were too viscous to be loaded onto the gel. Both images are from the same gel but were separated for presentation.
Thermo Scientific Pierce Universal Nuclease for Cell Lysis purity is comparable to that of other commercial nucleases. Pierce Universal Nuclease for Cell Lysis (1) and competitor E (2) were resolved by SDS-PAGE at a concentration of 3 μg (A) or 8 μg (B). Band intensities were stained with Imperial Protein Stain.
