Thermo Scientific Pierce Phosphatase Inhibitor Tablets provide complete cell lysate and biological sample protection from dephosphorylation by a broad spectrum of serine and threonine phosphatases.
The Pierce Phosphatase Inhibitor Tablets protect phosphoproteins from serine/threonine phosphatases and tyrosine phosphatases. Each Phosphatase Inhibitor Tablet contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate for broad-spectrum inhibition of phosphatase activity. Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10mL of solution. The Pierce Phosphatase Inhibitor Tablets are suitable for use with any cell or tissue extract to preserve protein phosphorylation for downstream applications.
- Economical – more cost effective than other commercially available formulations
- Easy-to-use – the refrigerator-stable tablets are easily dissolved in buffers and lysis reagents
- Complete – the tablets inhibit acid, alkaline, and protein phosphatases
- Compatible – use with homebrew or commercially available cell lysis reagents and Thermo Scientific Protein Assays
Protein phosphorylation and dephosphorylation states often function as molecular on/off switches for key regulators in biological pathways within the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation signaling events, endogenous phosphatases must be inactivated to prevent their uncontrolled activity upon cell lysis. Pierce Phosphatase Inhibitor Tablets preserve phosphoprotein modifications by significantly reducing endogenous acid, alkaline, and protein phosphatase activity.
Pierce Phosphatase Inhibitor Tablets are compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. Where incompatibilies arise, the inhibitor components can be removed by dialysis or desalting before performing these procedures.
To use Pierce Phosphatase Inhibitor Tablets, simply remove 1 tablet from the vial and dissolve in 10mL of buffer or lysate. The phosphatase inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Thermo Scientific Pierce Cell Lysis Solutions. The Pierce Phosphatase Inhibitor Tablets do not contain protease inhibitors. If necessary, protease inhibitors, such as Thermo Scientific Pierce Protease Inhibitor Tablets, can be added along with the phosphatase inhibitor tablet.
|Thermo Scientific Pierce Phosphatase Inhibitors preserve protein phosphorylation from phosphatases in cell and tissue extracts. Relative levels of total and phosphorylated protein were determined following protein extraction in the absence (–) or presence (+) of phosphatase inhibitors for (A.) AKT and PDGFR in serum-starved, PDGF-stimulated (100ng/mL) NIH 3T3 cell extracts and (B.) ERK1/2 in liver and spleen tissue extracts C. The degree of inhibition for protein, acid and alkaline phosphatase activity was determined in mouse brain extract following treatment with Pierce Phosphatase Inhibitor Tablets or another commercially available phosphatase inhibitor tablet.
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Pierce Cell Lysis Handbook
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