DyLight Green-emitting Specialty Dye

Label peptides, antibodies and other proteins at primary amines with the rhodamine-based, water-soluble Thermo Scientific DyLight Green-emitting Specialty Dye, which is comparable to Alexa Fluor 532.

DyLight Green-emitting Dye is a labeling agent that provides bright fluorescence detection for imaging. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetration in vivo, while more hydrophilic dyes have less nonspecific binding potential. This dye contains 2 sulfonates for increased solubility and an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.

Highlights:

• NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
• Water solubility – 2 sulfonate groups increase the solubility of this dye over over less-sulfonated dyes

Applications:

• Imaging
• Antibody labeling
• Direct immunofluorescence staining
• Flow cytometry
• Fluorescence correlation spectroscopy
• ELISA
• Western blotting
• Protein microarrays
• Polymer labeling
• Peptide labeling
• Phalloidin labeling for actin staining

Product Details:

Related Resources:

A review of fluorescent labeling
A review of chemical crosslinking

Related Products:

DyLight 543Q-NHS Ester and 641Q-NHS Ester (complementary fluorescence quenchers)
DyLight 488 Amine-Reactive Dye and Kits