The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.
Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates. Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate.
- Highly sensitive detection of firefly luciferase activity
- Use with Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal)
- Working solution stable for up to 2 months at -20°C
- Assay reagents compatible with other firefly luciferases
- Amenable to automation
- Contains a universal cell lysis buffer and optimized flash assay reagent
- Safe non-radioactive assay
Cell lysis buffer, reaction buffer and substrate; purchase Luciferase Signal Enhancer separately.
- Promoter studies for analyzing cis- regulatory elements and trans-acting factors
- Drug screening
- siRNA and miRNA screening
- Multiplexed assays to study off-target effects
- Secretory pathway / protein localization reporter assays
- Signal transduction pathway analysis
- RNA splicing studies
Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.
||Firefly luciferase reaction.
||Thermo Scientific Firefly Flash Assay Reagent and Luciferases deliver the brightest light intensity. HeLa cells were transfected with Thermo Scientific pCMV-Red Firefly Luc Vector (Red) or equivalent competitor vectors (Native and Promega* Gene, respectively). At 72 hours post-transfection, the indicated flash assay reagents were added to sample wells containing cell lysates. Total light output (RLU) was captured by Thermo Scientific Varioskan Flash Multimode Reader (integration time = 0.1 sec/reading). In combination with our vector and Signal Enhancer, the Thermo Scientific Pierce Firefly Flash Assay Kit provided the strongest bioluminescent signal.
Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide
pMCS-Red Firefly Luc Vector
Pierce Firefly Signal Enhancer
Pierce Luciferase Cell Lysis Buffer (2X)
TurboFect in vitro Transfection Reagent
Modified Dulbecco’s PBS BupH Packs
Modified Dulbecco’s PBS, 20X Solution
Firefly Luciferase Glow Assay Kit