In Vitro Protein Expression Systems

Vectors, plasmid controls and cell extracts for high-fidelity, cell-free in vitro translation, recombinant protein expression and post-translational modification from RNA or DNA templates.

Joshua LaBaer discusses using the HeLa lysates in Thermo Scientific Human IVT kits to search for biomarkers that can be used for early cancer detection

Selection Guides – Choose a product for your expression experiment:
- Pierce In Vitro Translation Systems
- Getting Started Guide – overview of critical parameters for successful human in vitro protein expression
Protein Methods Library – Learn more about protein expression:
- Overview of Protein Expression
- Cell-free Protein Expression
Related Product Categories – Other products of interest:
- Ni-NTA and cobalt IMAC resins for His-tagged protein purification
- Metabolic labeling reagents for Staudinger ligation
- Anti-TurboGFP Polyclonal Antibody (Part No. PA5-22688)


	1-Step Human Coupled In Vitro Expression Kits Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.


	1-Step Human High-Yield In Vitro Expression Kits Express 10 to 100 times more functional protein than other eukaryotic in vitro translation (IVT) kits using these cell-free kits with continuous-feed mini dialysis devices.


	1-Step Heavy Protein IVT Kit Express stable isotope-labeled (i.e., heavy) proteins by in vitro translation (IVT) for mass spectrometry using these cell-free expression kits.


	T7 Vectors for Cell-free Protein Expression Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.


	Custom Expression Vector Construction Service Custom expression vector construction services are available for inserting one or more cDNAs into an expression plasmid that is optimized for use with the Thermo Scientific Pierce Human In Vitro Protein Expression System.