Obtain the benefits of metal-enhanced DAB in a complete kit for immunohistochemical (IHC) staining.
Thermo Scientific Pierce Peroxidase IHC Detection Kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counterstain and preserve experimental results with frozen or paraffin tissue sections.
This immunohistochemical staining kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counter-stain and preserve experimental results with frozen or paraffin tissue section that have been probed with peroxidase enzyme conjugates. The Pierce Peroxidase IHC Detection Kit eliminates the need to procure numerous reagents from various sources, resulting in a time- and cost-effective way to perform peroxidase-based tissue staining. Harris Hematoxylin nuclear stain and mounting medium allow counter-staining and tissue preservation, respectively. The detection method is compatible with direct or avidin-biotin-complex (ABC) probing strategies.
Highlights:
Incredibly sensitive – fifty times more sensitive than the traditional DAB (3,3'-diaminobenzidine) method and 30 times more sensitive than other metal-intensified versions of DAB
Low background, high intensity, large dynamic range – gives a crisp dark brown-black precipitate that's more intense than the dull brown precipitate observed when using DAB without enhancement; substrate delivers increased intensity with almost nonexistent background
Easy-to-use, two-component system – mix the two liquid components and the working solution is ready-to-use; sufficient substrate and other staining reagents to treat up to 500 slides
Six-hour working solution stability – unlike other DAB substrates that must be used immediately, the working solution is stable for more than six hours at room temperature
Complete kit – saves time in reagent ordering, gathering and validating reagents for the IHC application; saves money compared to purchasing of all components separately
Applications:
Tissue immunohistochemistry procedures designed for DAB precipitating substrate
DAB staining of fixed paraffin sections, smears and frozen section preparations
Immunohisto- or immunocyto-staining using the metal-enhanced DAB substrate
A.
B.
Staining of Glial Fibrillary Acid Protein (GFAP) using the Pierce Peroxidase IHC Detection Kit. Panel A.Staining was performed as indicated in the protocol provided with the kit. Anti-GFAP and goat anti-mouse IgG biotin conjugate were used as the primary and secondary antibodies respectively. Streptavidin-HRP was the detection conjugate. Panel B. Same as Panel A except no anti-GFAP primary antibody was used.
Sufficient material to stain 250-500 tissue sections. Kit contains: DAB/Metal Concentrate (10X), 25 ml Universal Blocker Blocking Buffer in TBS, 250 ml Peroxidase Suppressor, 2 x 100 ml Stable Peroxide Buffer, 250 ml BupH Tris Buffered Saline, 4 packs Surfact-Amps 20 (10% Tween-20), 10 ml Harris Modified Hematoxylin, 100 ml Mounting Medium, 60 ml
