Microplate kits and reagent sets for sandwich ELISA of pig TNF alpha.
Assay TNFa (porcine tumor necrosis factor alpha) levels in pig serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Porcine TNF alpha ELISA Kits.
These porcine TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα; rat TNFα; bovine TNFα. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%.
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
Porcine TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Porcine TNF alpha ELISA Kit (Part No. EP2TNFA), with error bars based on four replicates for each tumor necrosis factor alpha standard concentration. Also see standard curve for the TNFα Reagent Kit.