Rat TNF alpha ELISA Kits

Assay TNFα (rat tumor necrosis factor alpha) levels in rat serum and culture supernatant samples with our Thermo Scientific Rat TNF alpha ELISA Kits.

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: natural and recombinant rat TNFα (rat tumor necrosis factor alpha)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 31 to 2500pg/mL
• Sensitivity: < 15pg/mL
• Precision: intra-assay CV < 10%; inter-assay CV < 10%
• Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3 hours
• Sample Size: 50μL
• Specificity: no significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%).

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Rat TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Rat TNF alpha ELISA Kit (Part No. ER3TNFA), with error bars based on four replicates for each tumor necrosis factor alpha standard concentration.

References:

• TNF alpha ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search TNF alpha Antibodies (www.pierce-antibodies.com) – matched pair links coming soon