Rat IL-10 ELISA Kits

Assay IL-10 (rat interleukin-10) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat IL-10 ELISA Kits.

These rat IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: natural and recombinant rat interleukin-10 (IL-10)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 16 to 500pg/mL
• Sensitivity: < 3pg/mL
• Precision: dependent upon sample type (generally less than 10% CV)
• Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3 hours
• Sample Size: 50μL
• Specificity: no significant cross-reaction with rat IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, MCP-1, MIP-1α, RANTES, TNFα, human IL-10, or mouse IL-10.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Rat IL-10 ELISA standard curve. An example standard curve for the Thermo Scientific Rat IL-10 ELISA Kit (Part No. ERIL10), with error bars based on six replicates for each interleukin-10 standard concentration.

References:

• IL-10 ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search IL-10 Antibodies (www.pierce-antibodies.com) – matched pair links coming soon