Microplate kits for sandwich ELISA of rat interleukin-1 beta (IL-1b).
Assay IL-1β (rat interleukin-1 beta) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat IL-1 beta ELISA Kits.
These rat IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant rat interleukin-1 beta (IL-1β)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Rat IL-1β ELISA standard curve. An example standard curve for the Thermo Scientific Rat IL-1β ELISA Kit (Part No. ER2IL1B), with error bars based on six replicates for each interleukin-1 beta standard concentration.