Rat IL-1 beta ELISA Kits

Assay IL-1β (rat interleukin-1 beta) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat IL-1 beta ELISA Kits.

These rat IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: natural and recombinant rat interleukin-1 beta (IL-1β)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 25.6 to 2500pg/mL
• Sensitivity: < 12pg/mL
• Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3.5 hours
• Sample Size: 50μL
• Specificity: no significant cross-reaction with rat IFNγ, IL-1α, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1α, or RANTES, or human, mouse or pig IL-1β.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Rat IL-1β ELISA standard curve. An example standard curve for the Thermo Scientific Rat IL-1β ELISA Kit (Part No. ER2IL1B), with error bars based on six replicates for each interleukin-1 beta standard concentration.

References:

• IL-1 beta ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search IL-1 beta Antibodies (www.pierce-antibodies.com) – matched pair links coming soon