Microplate kits and reagent sets for sandwich ELISA of mouse TNF alpha.
Assay TNFα (mouse tumor necrosis factor alpha) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse TNF alpha ELISA Kits.
These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Specificity: no significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNγ or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants.
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).
Mouse TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Mouse TNF alpha ELISA Kit (Part No. EMTNFA), with error bars based on six replicates for each tumor necrosis factor alpha standard concentration. Also see standard curve for the TNFα Antibody Pair Kit.
TNFα, also called cachectin, macrophage cytotoxin, necrosin, etc., is produced primarily by macrophages in response to bacteria, viruses, mycoplasma, immune complexes, other cytokines, tumor cells, complement and phorbol esters among others. It is a 157 amino acid long polypeptide with an apparent MW of 17 kDa under denaturing conditions but exists as a 45-55 kDa trimer naturally.
In vivo, TNFα has anti-tumor activities against some solid tumors, induces tumorigenesis, metastisis; acts in immunomodulation; induces septic shock, fever; plays a role in multiple sclerosis; and is involved in autoimmunity and infectious disease. TNFα alone and in combination with other cytokines and chemical agents has been used as an anti-tumor agent. Patients with melanoma and other solid tumors, administered high doses of TNFα, have realized a cure rate of up to 90% albeit with some major and minor side effects.