Microplate and reagent kits for sandwich ELISA of mouse MCP-1.
Assay MCP-1 (mouse monocyte chemoattractant protein-1) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse MCP-1 ELISA Kits.
These mouse MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1α, RANTES or TNFα, or human or rat MCP-1.
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
Mouse MCP-1 ELISA standard curve. An example standard curve for the Thermo Scientific Mouse MCP-1 ELISA Kit (Part No. EH2MCP1), with error bars based on four replicates for each MCP-1 standard concentration. Also see standard curve for the MCP-1 Reagent Kit.