Microplate kits for sandwich ELISA of total mouse interleukin-12 (IL-12, p70 plus p40).
Assay IL-12 p40 + p70 (total mouse interleukin-12) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (total) ELISA Kits.
These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Target: total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 47 to 3000pg/mL
Sensitivity: < 12pg/mL
Precision: dependent upon sample type (generally, intra- and inter-assay CV < 10%)
Mouse total IL-12 (p70 + p40) ELISA standard curve. An example standard curve for the Thermo Scientific Mouse Total IL-12 ELISA Kit (Part No. EMIL12TOT), with error bars based on six replicates for each interleukin-12 standard concentration.