Microplate kits and reagent sets for sandwich ELISA of mouse interleukin-4 (IL-4).
Assay IL-4 (mouse interleukin-4) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse IL-4 ELISA Kits.
These mouse IL-4 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-4 antibody pairs and IL-4 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant mouse interleukin-4 (IL-4)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with with mouse IL-2, IL-3, IL-5, IL-6, IL-7, IFNγ, GM-CSF, TNFα; rat IL-4; rabbit IL-4; or human IL-4.
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).
Mouse IL-4 ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IL-4 ELISA Kit (Part No. EMIL4), with error bars based on twelve replicates for each interleukin-4 standard concentration. Also see standard curves for the IL-4 Antibody Pair Kit.