Mouse IL-1 alpha ELISA Kits

Assay IL-1α (mouse interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-1 alpha ELISA Kits.

These mouse IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: natural and recombinant mouse interleukin-1 alpha (IL-1α)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 15.6 to 1000pg/mL
• Sensitivity: < 6pg/mL
• Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3 hours
• Sample Size: 50μL
• Specificity: no significant cross-reaction with human IL-1α, human IL-1β, or mouse IL-1β, IL-3, IL-4, IL-5, IL-7, IL-10, TNFα, IFNγ, or GM-CSF.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Mouse IL-1α ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IL-1α ELISA Kit (Part No. EMIL1A), with error bars based on six replicates for each interleukin-1 alpha standard concentration.

References:

• IL-1 alpha ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search IL-1 alpha Antibodies (www.pierce-antibodies.com) – matched pair links coming soon