Microplate kits and reagent sets for sandwich ELISA of mouse interferon gamma (IFN-g).
Assay IFN-γ (mouse interferon gamma) levels in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Mouse IFN gamma ELISA Kits.
These mouse IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant mouse interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with with mouse IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFα or human IFNα or IFNγ
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).
Mouse IFNγ ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IFNγ ELISA Kit (Part No. EM1001), with error bars based on five replicates for each interferon gamma standard concentration. Also see standard curves for the IFNγ Reagent Kit and IFNγ Antibody Pair Kit.