Human MCP-1 ELISA Kits

Assay MCP-1 (human monocyte chemoattractant protein-1) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human MCP-1 ELISA Kits.

These human MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: natural and recombinant human MCP-1 (monocyte chemoattractant protein-1)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 25.6 to 1000pg/mL
• Sensitivity: < 10pg/mL
• Precision: intra-assay CV < 10%; inter-assay CV < 10%
• Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3 hours
• Sample Size: 50μL
• Specificity: no significant cross-reaction with human Angiogenin, EGF, FGF-basic, Eotaxin, GM-CSF, GROα, HB-EGF, HGF, IFNα, IFNγ, KGF, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, PLGF-1, RANTES, TNFα, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, and IL-17.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human MCP-1 ELISA standard curve. An example standard curve for the Thermo Scientific Human MCP-1 ELISA Kit (Part No. EH2MCP1), with error bars based on twelve replicates for each MCP-1 standard concentration.

References:

• MCP-1 ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search MCP-1 Antibodies (www.pierce-antibodies.com) – matched pair links coming soon