Human IL-12 (total) ELISA Kits

Assay IL-12 p40 + p70 (total human interleukin-12) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with these hermo Scientific Human IL-12 (total) ELISA Kits.

These human IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, HRP-conjugate, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: total (p70 and p40) natural and recombinant human interleukin-12 (IL-12)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 25.6 to 1000pg/mL
• Sensitivity: < 5pg/mL
• Precision: intra-assay CV < 10%; inter-assay CV < 10%
• Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3 hours
• Sample Size: 50μL
• Specificity: no significant cross-reaction with human GM-CSF, IFNα, IFNγ, TNFα, TNFβ, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, mouse IL-12 (p70) or mouse IL-12 (p40).

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human total IL-12 (p70 + p40) ELISA standard curve. An example standard curve for the Thermo Scientific Human Total IL-12 ELISA Kit (Part No. EH2IL12T), with error bars based on four replicates for each interleukin-12 standard concentration.

References:

• IL-12 ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search IL-12 Antibodies (www.pierce-antibodies.com) – matched pair links coming soon