Microplate kits for sandwich ELISA of biologically active human interleukin-12 (IL-12 p70).
Assay IL-12 p70 (active human interleukin-12, p70) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-12 (p70) ELISA Kits.
These human IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically active interleukin-12 (IL-12 p70) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Target: biologically active (p70) natural and recombinant human interleukin-12 (IL-12, p70)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, TNFα, IFNγ, GM-CSF, GROα or GROβ. Recombinant human IL-12 (p40) and mouse IL-12 (p40) do not cross-react or interfere with this assay.
Human active IL-12 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-12 p70 ELISA Kit (Part No. EHIL12), with error bars based on four replicates for each biologically active interleukin-12 p70 standard concentration.