Human IL-5 ELISA Kits

Assay IL-5 (human interleukin-5) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-5 ELISA Kits.

This human IL-5 receptor ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble interleukin-5 (IL-5) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: natural and recombinant human interleukin-5 (IL-5)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 0 to 400pg/mL
• Sensitivity: < 2pg/mL
• Precision: intra-assay CV < 10%; inter-assay CV < 10%
• Sample Types: cell culture supernatant, urine serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3 hours
• Sample Size: 50μL
• Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, TNFα, IFNγ, IFNα, GM-CSF, GROα, GROβ, or mouse IL-5.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Human IL-5 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-5 ELISA Kit (Part No. EHIL5), with error bars based on six replicates for each interleukin-5 standard concentration.

References:

• IL-5 ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search IL-5 Antibodies (www.pierce-antibodies.com) – matched pair links coming soon