High-sensitivity Southern blotting without radioactivity.
Thermo Scientific North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.
The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.
Highlights:
Sensitivity equal to or greater than 32P
Much faster than DIG detection systems
Amenable to nearly any DNA or RNA hybridization method
Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
Specific plaques can be picked out of areas that are very dense with signal due to extremely low background
Product Details:
Detection of biotinylated DNA on a Southern blot using the North2South System and a cooled CCD imaging system. A Southern blot of biotinylated DNA samples from a DNA fingerprinting kit was detected using the North2South System and imaged for one minute using a cooled CCD camera system.
References:
Adilakshmi, T. and Laine, R.O. (2002). J. Biol. Chem.277(6), 4147-4151.
Bach, S., et al. (2002). Infect. Immun.70(2), 988-992.
Borst, E-M., et al. (1999). J. Virol.73(10), 8320-8329.
Haertel-Wiesmann, M., et al. (2000). J. Biol. Chem.275(41), 32046-32051.
