Thermo Scientific EZ-Link Amine-PEG2-Biotin and Amine-PEG3-Biotin are water-soluble biotin compounds containing polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods.
Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the –NH2 group of the amino-biotin, forming an amide bond.
Highlights:
- Biotinylation – label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
- Amine-activated – primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
- Pegylated – polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules
- Medium length – spacer arms (total lengths added to target) are 20.4 and 22.9 angstroms, providing slightly different options for controlling steric hindrance and hydrophilicity
Product Details:
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| Chemical structures of Amine-PEG-Biotins. These biotinylation reagents are often conjugated to carboxyl groups using the crosslinker EDC. For more information, see our review of Carbodiimide Reaction Chemistry. |
| Properties of Amine-PEG-Biotin Compounds. Also see Amine-PEG11-Biotin. |
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Amine-PEG2-Biotin
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Amine-PEG3-Biotin
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| Alternative names |
Biotin-PEO2-Amine
Biotin-dPEG2-Amine |
Biotin-PEO3-Amine
Biotin-dPEG3-Amine |
| Chemical name |
(+)-biotinyl-3,6-dioxaoctanediamine |
(+)-biotinyl-3,6,9,-trioxaundecanediamine |
| Molecular formula |
C16H30N4O4S |
C18H34N4O5S |
| Molecular weight |
374.50 |
418.55 |
| Spacer arm length |
20.4 angstroms |
22.9 angstroms |
| Mass added to target |
356.19 daltons |
400.21 daltons |
| Form |
White to off-white solid |
White to off-white solid |
| Solubility |
At least 25mg/mL in water |
At least 25mg/mL in water |
| Storage conditions |
4°C |
4°C |
| SMILES structures |
NCCOCCOCCNC(CCCCC1C(C(CS1)N2)NC2=O)=O
O=C1NC2C(C(CCCCC(NCCOCCOCCOCCN)=O)SC2)N1 |
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using EDC carbodiimide crosslinker chemistry. Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22890), carboxylates react with amino (–NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry).
References:
- Bronfman, F.C., et al. (2003) J. Neurosci.. 23, 3209-3220.
- Hara, T., et al. (2003) J. Biol. Chem. 278, 40408-40414.
- Pihlajamaa, T., et al. (2004) J. Biol. Chem.279, 24265-24273.
Related Resources:
Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)
Review of PEGylation
Related Products:
EDC Crosslinker
Sulfo-NHS
Amine-PEG11-Biotin
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins
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