Thermo Scientific Pierce APTS (3-aminopropyltriethoxysilane) is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules.
3-Aminopropyltriethoxysilane (APTS) is a surface treatment used to chemically couple various ligands to glass or silica surfaces such as glass slides. 3-aminopropyltriethoxysilane is first coupled to glass or silica through the silane. Compounds of interest are then coupled to the newly added amino groups directly, or additional chemistries are applied using the amino function prior to coupling.
Highlights:
- Crosslinking agents such as BS3, EDC and Sulfo-SMCC can be used to immobilize proteins, peptides, modified DNA, etc., to the treated surface
- Coats certain plastic surfaces noncovalently to create a reactive film
- Chemically adheres tissue sections to glass slides for immunohistochemistry
Related Resources:
Overview of Immunohistochemistry (Protein Methods Library article)
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