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 Thermo Scientific SDS-Out SDS Precipitation Reagent provides convenient removal of SDS from small-volume protein samples (0.1 to 10mL).
The simple reagent and kit effectively remove excess SDS from small protein samples. Sodium dodecyl sulfate (SDS) is a detergent typically used in cell lysis reagents and other protein solubilization buffers. Traditional methods for SDS removal include prolonged dialysis, anion exchange chromatography, affinity columns or and acetone precipitation. By comparison, the SDS-Out Precipitation Reagent is relatively easy to use and provides excellent protein recovery.
Highlights:
- Convenient removal of SDS from microliter-volume samples
- Use for samples containing 0.1 to 1% SDS
- Minimal dilution of small-volume samples
- Protein recovery is 90 to 100% (based on tests with BSA, cytochrome C, soybean trypsin inhibitor, ovalbumin, ribonuclease A, myoglobin and human IgG)
Product Details:
| Excellent protein recovery after removal of SDS. 0.5mL samples of protein solution each at a concentration of 1mg/mL were used to develop this table.Values are percent protein recovery from protein solutions with increasing SDS concentrations (0.125% to 1%) upon removal fo the SDS with the Thermo Scientific SDS-Out SDS Precipitation Reagent. |
| Protein † |
Protein Recovery |
| Bovine Serum Albumin |
97.5% |
98.0% |
98.4% |
100% |
| Cytochrome C |
100% |
100% |
100% |
100% |
| Soybean Trypsin Inhibitor |
99.0% |
98.5% |
98.8% |
100% |
| Ovalbumin |
99.8% |
99.6% |
98.1% |
93.8% |
| Ribonuclease |
100% |
100% |
100% |
100% |
| Myoglobin |
100% |
100% |
100% |
97.9% |
| Human IgG |
100% |
92.7% |
88.7% |
76.2% |
| Initial Concentration of SDS |
0.125% |
0.25% |
0.5% |
1.0% |
Related Resources:
Review of sample preparation for mass spectrometry
Review of detergents for Cell Lysis
Related Products:
Pierce Detergent Removal Spin Columns
Browse Thermo Scientific Pierce Detergents
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